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The substrate solution was then stored at −twenty°C till the ammonium and nitrate contents have been analyzed Along with the San++ Continual-Flow Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate resolve was dependant on a response with hydrazinium sulfate, sulfanilamide and N-(one-naphthyl) ethylenediamine dihydrochloride which resu